Simultaneous estimation of amoxicillin trihydrate and dicloxacillin sodium in capsule

Abstract

Amoxicillin trihydrate and dicloxacillin sodium were simultaneously determined using RP-HPLC in bulk and formulations using a simple, fast, precise, selective and accurate method. Water and potassium dihydrogenortho phosphate buffer were used to achieve isocratic separation on a C18 column. The flow rate was 1.2ml\minute and effluent were detected at 225nm.The retention time of amoxillintrihydrate and dicloxacillin sodium were. 943 mins and 11. 678 mins respectively. There was linearity between 0. 999 and 0. 999, with correlation coefficients of 0. 9998 and 0. 9995. Percent recoveries obtained for both the drugs were 98. 45-100.65% and 98.79 - 100.01% respectively. linearity, accuracy, robustness, specificity and precision of the method were validated according to ICH guidelines. Analyzing amoxicillin trihydrate & dicloxacillin sodium in their combined dosage form can be routinely done using the method developed.